人工培养柞蚕蝉花不同部位的代谢组差异

【背景】蝉花是传统中药材,前期研究发现柞蚕培养蝉花与野生蝉花成分类似,因此有替代野生蝉花利用的潜力。【目的】揭示柞蚕培养蝉花各部分的代谢组差异,为合理利用人工培养柞蚕蝉花提供理论依据。【方法】用基于HPLC-DAD-TOF/MS代谢组学方法,比较人工培养柞蚕蝉花的虫体、体表菌丝和孢梗束的代谢组差异。【结果】人工培养柞蚕蝉花不同部位代谢组差异显著。蝉花虫体内虽然充满菌丝(菌核),但次生代谢产物并不多,仅二氢神经酰胺和植物鞘胺醇等少数化合物较其他部位多,表明虫体内物质以大分子储藏物质为主。虫体内的植物鞘胺醇含量较高,与蝉棒束孢受柞蚕蛹的免疫损伤有关。虫体表面菌丝中被检测到大量次生代谢产物,这可能是因为虫体内分解的营养成分会优先被菌丝吸收。孢梗束的次生代谢产物相对较少。【结论】柞蚕蝉花不同部位的代谢物显著不同,其虫体表面菌丝次生代谢产物较多,具有较大的代谢物利用潜力。

Metabolomic differences among different parts of Isaria cicadae cultured on Antheraea pernyi

Background] Isaria cicadae is a Chinese traditional medicine. A preliminary study showed that wild I. cicadae contains similar metabolites with cultured I. cicadae on Antheraea pernyi. Therefore, the cultured I. cicadae has the potential to be used as an alternative. Objective] Reveal the metabolites difference between different parts of the cultured I. cicadae. The results will provide a theoretical basis for the rational use of the cultured I. cicadae. Methods] HPLC-DAD-TOF/MS-based metabolomics was used to compare the metabolic difference between the insect body part, mycelia on the insect surface and coremia of I. cicadae. Results] Different parts of cultured I. cicadae showed significantly different metabolomics. Despite that, the insect part is full of mycelia (sclerotia) which contains less small molecular metabolites than the other parts, and has only a relatively higher level of dihydroceramide and phytosphingosine. This result implied that it contained mainly insoluble macromolecular substances. The high level of phytosphingosine was possibly related to immune injury from the host insect. Lots of small molecular metabolites were detected from mycelia on the surface of the insect body implying that nutrients from the insects were preferentially absorbed by the mycelia. The coremia contain relatively few secondary metabolites. Conclusion] Different parts of cultured I. cicadae on A. pernyi contain significantly different metabolites, and mycelia on the surface of the insect contain relatively higher level of secondary metabolites suggesting more potential of metabolites utilization.