亚洲玉米螟交配率和交配次数与其日龄、性比和精巢大小的关系

【目的】昆虫交配行为与其日龄和生殖系统发育密切相关。本研究旨在明确亚洲玉米螟Ostrinia furnacalis交配行为与其日龄、性比和雄蛾精巢大小的关系,为性信息素群集诱杀和交配干扰防控亚洲玉米螟提供理论依据。【方法】通过室内交配实验测定了不同配对晚数、日龄、性比亚洲玉米螟的交配率和交配次数,通过雄蛾精巢解剖分析了交配次数与精巢体积的关系。【结果】亚洲玉米螟的交配率随配对晚数增加而增加,但第1-5晚的增加速度大于第6-7晚的;1-4日龄蛾的交配率随日龄增加而增加,5日龄蛾的交配率显著降低;1-3日龄蛾配对1晚的交配率与新羽化蛾配对2～4晚的均无显著差异。亚洲玉米螟可以交配1～3次,但是交配3次的比例极低。雌性数量从1增为2时,雄蛾的交配率显著增加;从2增为3时,雄蛾的交配率显著降低、死亡率显著增加,交配1次的雄蛾进行二次交配的比例也增加,并且雄蛾更愿意与处女雌蛾进行第2次交配;交配1次雄蛾的精巢体积总是显著大于同期未交配雄蛾的,交配1次稍降低了雄蛾精巢的衰退速度,交配2次则明显增加雄蛾精巢的衰退速度。【结论】本研究明确了亚洲玉米螟交配与日龄、性比和雄蛾精巢体积之间的关系,从理论上证实了性信息素群集诱杀技术和交配干扰技术防治亚洲玉米螟的有效性。

The relationship between the mating rate and frequency of the Asian corn borer,Ostriniafurnacalis(Lepidoptera: Pyralidae) and its age,sex ratio and testicular volume

【Aim】 The mating behavior of insects is closely related to their age and thedevelopment of reproductive system. This study aims to explore the relationship between themating activity and age, sex ratio and the testicular volume of male moths of the Asiancorn borer (ACB), Ostrinia furnacalis, so as to provide the theoretical basis for theapplication of ACB sex pheromone in mass trapping and mating disruption in the field.【Methods】 The mating rate and frequency of ACB were determined by mating experiments inthe laboratory with different pairing nights, age and sex ratios, and the relationshipbetween the testicular volume and the mating frequency of male moths was analyzed bytesticular anatomy. 【Results】 The mating rate of ACB increased with the number of pairingnights, but the increase was more rapid from the 1st to the 5th night than that from the6th to the 7th night. The mating rate of moths increased with the age from 1-day-old to 4-day-old, but the mating rate of the 5-day-old moths decreased significantly. There wasno significant difference in the mating rate between the 1-3 day-old moths pairing onenight and pairing 2-4 nights after eclosion. ACB could mate 1-3 times, but the proportionof moths mating thrice was very low. When the number of pairing female moths increased from1 to 2, the mating rate of male moth increased significantly. However, when the number offemale moths increased to 3, the mating rate of male moth decreased significantly, themortality rate of male moths increased significantly, and the proportion of moths matingtwice increased. Male moth preferred to mate with virgin female for its second mating. Thetesticular volume of male moths mating once was always significantly larger than that ofthe unmated males at the same day-old, and the testicular shrinkage of males was slightlydelayed by mating. Mating twice significantly increased the testicular shrinkage of malemoths. 【Conclusion】 This study clarifies the relationship between the mating of ACB andthe age, the sex ratio and the testicular volume of male moth. The results provide atheoretical basis for the development of mating disruption and mass trapping by sexpheromone and confirm the effectiveness of its application in the field.