Some Aspects of Growth and Metabolism of Paul's Scarlet Rose Cell Suspensions

The growth pattern of suspension cultures of Paul’s Scarletrose cells has been examined. No distinct phases of cell divisionand cell expanison could be recognized. The 2-day lag phaseobserved after inoculation of stationary-phase cells into freshmedium was followed by a rapid entry into exponential growthduring which a mean cell-generation time of 36 h was recorded.Metabolic development assessed in terms of respiratory activityand RNA, DNA, and protein accumulation has been related to thephases of the growth cycle. A high metabolic activity developsimmediately after inoculation reaching a peak by early-exponentialphase. This activity sustains exponential growth until factorsin the medium become limiting. The pattern of DNA accumulationis closely correlated with increase in cell number and freshweight whereas changes in RNA levels are accompanied by similarchanges in protein levels and respiratory activity. The accumulationof phenolics follows a pattern unlike those of the other parametersexamined.