The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins |
| |
Authors: | Pantaloni D Boujemaa R Didry D Gounon P Carlier M F |
| |
Institution: | Laboratoire d'Enzymologie et Biochimie Structurale, CNRS, Gif-sur-Yvette, France. |
| |
Abstract: | The Arp2/3 complex is an essential regulator of actin polymerization in response to signalling and generates a dendritic array of filaments in lamellipodia. Here we show that the activated Arp2/3 complex interacts with the barbed ends of filaments to initiate barbed-end branching. Barbed-end branching by Arp2/3 quantitatively accounts for polymerization kinetics and for the length correlation of the branches of filaments observed by electron microscopy. Filament branching is visualized at the surface of Listeria in a reconstituted motility assay. The functional antagonism between the Arp2/3 complex and capping proteins is essential in the maintenance of the steady state of actin assembly and actin-based motility. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |