基于16S rRNA高通量测序技术分析福州地区慢传输型便秘患者肠道菌群的变化

运用16S rRNA高通量测序技术分析福州地区慢传输型便秘(STC)患者肠道菌群变化的特征。

纳入60例STC患者和健康志愿者20例, 留取新鲜粪便样本, 冰块运送至实验室并存放于-80度冰箱, 应用16S rRNA高通量测序技术, 分析各组肠道菌群的生物多样性与结构组成。

测序后共得到1 702 004 524条有效序列, 样本序列平均有效长度为414.1 bp, 总样本平均序列条数为2 127 505.7条。与对照组比较, STC患者肠道菌群丰度指数Ace指数、Chao指数和肠道菌群多样性指数Shannon指数下降(P < 0.05), 肠道菌群多样性指数Simpson指数显著升高(P < 0.05)。在门水平上, STC组以拟杆菌门、放线菌门为主, 正常对照组以厚壁菌门为主。在属水平上, STC组以拟杆菌属(Bacteroides)、肠杆菌科(Enterobacteriaceae)、丹毒杆菌属(Erysipelatoclostridium)、巨单胞菌属(Megamonas)为主, 正常对照组以乳酸杆菌属(Lactobacillus)、双歧杆菌属(Bifidobacterium)、布劳特菌属(Blautia)为主。

STC患者肠道菌群发生紊乱, 表现为丰度及多样性下降, 菌群结构发生改变, 可能与慢传输型便秘的发生发展有关。

Intestinal flora in patients with slow transit constipation in Fuzhou based on 16SrRNA high-throughput sequencing technology

ObjectiveTo analyze the characteristics of intestinal flora changes in patients with slow-transit constipation(STC) in Fuzhou using 16 S rRNA high-throughput sequencing technology.MethodsSixty STC patients and 20 healthy volunteers were enrolled, and fresh stool samples were collected, transported on ice to the laboratory and stored at-80 ℃. The biodiversity and structural composition of the intestinal flora in each group were analyzed with 16 S rRNA high-throughput sequencing technology.ResultsA total of 1 702 004 524 valid sequences were obtained, with an average effective length of 414.1 bp and an average number of 2 127 505.7 sequences in the total samples. Compared with the control group, the intestinal flora abundance indexes(Ace index, Chao index) and intestinal flora diversity index(Shannon index) decreased(P < 0.05), while Simpson index increased significantly(P < 0.05) in STC patients. At the phylum level, the flora in STC group was dominated by Bacteroides and Actinobacteria, and in the normal control group by Firmicutes. At the genus level, the flora in STC group was dominated by Bacteroides, Enterobacteriaceae, Erysipelatoclostridium and Megamonas, while in the normal control group by Lactobacillus, Bifidobacterium and Blautia.ConclusionThe intestinal flora of patients with slow transit constipation is disordered, which is manifested by decreased abundance and diversity, changes in the structure of the bacterial community, decreased beneficial bacteria, and increased pathogenic bacteria, and which may be related to the occurrence and development of slow transit constipation.