Muscle Differentiation in Cultured Pineal Body Cells of Neonatal Rats |
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| Authors: | K. WATANABE H. AOYAMA Y. NOJYO T. MATSUURA T. S. OKADA |
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| Affiliation: | Department of Anatomy. Fukui Medical School, Matsuoka-cho, Yoshida-gun, Fukui 910-11, Japan;Institute of Biophysics, Faculty of Science, University of Kyoto, Kyoto 606, Japan;Department of Anatomy. Fukui Medical School, Matsuoka-cho, Yoshida-gun, Fukui 910-11, Japan;Department of Anatomy, Kyoto Prefectural University of Medicine, Kawara-machi, Kamigyo-ku, Kyoto 602, Japan;Institute of Biophysics, Faculty of Science, University of Kyoto, Kyoto 606, Japan |
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| Abstract: | Dissociated cells of pineal bodies of new-born rats were cultured to see what cell types would be differentiated during culture in vitro for about 4 weeks. In early stages of culture, about 10 days after inoculation, flattened cells with piliform processes, small round cells and small bubbling cells were distinguishable in the cultures. After about 2 weeks, neuronal cells with axon-like processes and multinuclear muscle-like cells were differentiated. On further culture, the latter cells differentiated into mature striated myotubes. The developmental origin of myotube formed in cell cultures of pineal body is discussed. |
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