Tungstate can substitute for molybdate in sustaining growth of Methanobacterium thermoautotrophicum |
| |
Authors: | Peter A Bertram Ruth A Schmitz Dietmar Linder Rudolf K Thauer |
| |
Institution: | (1) Max-Planck-Institut für Terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, D-35043 Marburg, Germany;(2) Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Karl-von-Frisch-Strasse, D-35043 Marburg, Germany;(3) Fachbereich Humanmedizin, Justus-Liebig-Universität, D-35392 Gießen, Germany |
| |
Abstract: | Methanobacterium thermoautotrophicum (strain Marburg) was found to grow on media supplemented with tungstate rather than with molybdate. The Archaeon then synthesized a tungsten iron-sulfur isoenzyme of formylmethanofuran dehydrogenase. The isoenzyme was purified to apparent homogeneity and shown to be composed of four different subunits of apparent molecular masses 65 kDa, 53 kDa, 31 kDa, and 15 kDa and to contain per mol 0.4 mol tungsten, <0.05 mol molybdenum, 8 mol non-heme iron, 8 mol acid-labile sulfur and molybdopterin guanine dinucleotide. Its molecular and catalytic properties were significantly different from those of the molybdenum isoenzyme characterized previously. The two isoenzymes also differed in their metal specificity: the active molybdenum isoenzyme was only synthesized when molybdenum was available during growth whereas the active tungsten isoenzyme was also generated during growth of the cells on molybdate medium. Under the latter conditions the tungsten isoenzyme was synthesized containing molybdenum rather than tungsten.Abbreviations MFR
methanofuran
- CHO-MFR
N-formylmethanofuran
- MGD
molybdopterin guanine dinucleotide
- MAD
molybdopterin adenine dinucleotide
- MHD
molybdopterin hypoxanthine dinucleotide
- FPLC
fast protein liquid chromatography
- SDS/PAGE
sodium dodecylsulfate/polyacrylamide gel electrophoresis
- ICP-MS
inductively coupled plasma mass spectrometry |
| |
Keywords: | Formylmethanofuran dehydrogenase Tungsten enzymes Molybdopterin dinucleotides Methanogenesis Archaea Archaebacteria Methanobacterium thermoautotrophicum Methanobacterium wolfei |
本文献已被 SpringerLink 等数据库收录! |
|