Spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell |
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Authors: | Kim Jung Rae Beecroft Nelli J Varcoe John R Dinsdale Richard M Guwy Alan J Slade Robert C T Thumser Alfred Avignone-Rossa Claudio Premier Giuliano C |
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Institution: | (1) Sustainable Environment Research Centre (SERC), Faculty of Advanced Technology, University of Glamorgan, Pontypridd, Mid-Glamorgan, CF37 1DL, UK;(2) Sustainable Environment Research Centre (SERC), Faculty of Health, Sport and Science, University of Glamorgan, Pontypridd, Mid-Glamorgan, CF37 1DL, UK;(3) Microbial Sciences, University of Surrey, Guildford, GU2 7XH, UK;(4) Biochemical Sciences, University of Surrey, Guildford, GU2 7XH, UK;(5) Chemical Sciences, University of Surrey, Guildford, GU2 7XH, UK; |
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Abstract: | The spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal
tubular microbial fuel cell reactors, consisting of two serially connected modules. The proportional changes in the microbial
community composition were assessed by polymerase chain reaction–denaturing gradient gel electrophoresis (DGGE) and DNA sequencing
in order to relate them to the performance and stability of the bioelectrochemical system. The reproducibility of duplicated
reactors, evaluated by cluster analysis and Jaccard’s coefficient, shows 80–90% similarity in species composition. Biofilm
development through fed-batch start-up and subsequent stable continuous operation results in a population shift from γ-Proteobacteria- and Bacteroidetes- to Firmicutes-dominated communities, with other diverse species present at much lower relative proportions. DGGE patterns were analysed
by range-weighted richness (Rr) and Pareto–Lorenz evenness distribution curves to investigate the evolution of the bacterial community. The first modules
shifted from dominance by species closely related to Bacteroides graminisolvens, Raoultella ornithinolytica and Klebsiella sp. BM21 at the start of continuous-mode operation to a community dominated by Paludibacter propionicigenes-, Lactococcus sp.-, Pantoea agglomerans- and Klebsiella oxytoca-related species with stable power generation (6.0 W/m3) at day 97. Operational strategies that consider the dynamics of the population will provide useful parameters for evaluating
system performance in the practical application of microbial fuel cells. |
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