粗提和纯化的HPV16L1重组蛋白为抗原的ELISA比较

为评价真核及原核细胞表达的HPV16L1粗提蛋白代替纯化的16L1蛋白作为ELISA检测抗原的可行性。实验分别用大肠埃希菌表达的HPV16L1纯化蛋白,表达16L1的大肠埃希菌破碎物及表达16L1的昆虫细胞破碎物作为抗原。在ELISA试验中与抗HPV16L1的单克隆抗体进行反应。结果证实3种包被抗原可以得出非常相近的OD值变化趋势。表明用表达16L1的大肠埃希菌破碎物和表达16L1的昆虫细胞破碎物可以代替纯化的HPV16L1蛋白作为抗原进行ELISA试验。

ELISA Experiment Comparison of Crude and Purified HPV16L1 Recombinant Protein as Antigen

The feasibility of replacement of purified HPV(human papillomavirus)16L1 proteins with crude ones as antigen in ELISA test was evaluated.The experiment respectively used E.coli expressed HPV16L1 purified protein, pieces of E.coli that expressed 16L1,and pieces of insect cell that expressed 16L1 as antigens.Serial dilutions of an- ti-HPV16L1 monoclonal antibody were used in the ELISA test.The results have proved that three different coating an- tigens could obtain very similar OD values changing trend.These indicated that pieces of E.coli and pieces of insect cell that expressed 16L1 proteins could replace purified HPV16L1 proteins as antigen in ELISA test.