Genetic diversity analysis of <Emphasis Type="Italic">Sclerotinia sclerotiorum</Emphasis> causing stem rot in chickpea using RAPD,ITS-RFLP,ITS sequencing and mycelial compatibility grouping |
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Authors: | A K Mandal Sunil C Dubey |
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Institution: | (1) Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India;(2) Present address: Directorate of Seed Research, Kushmaur, Kaithauli, Mau, 275101, Uttar Pradesh, India |
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Abstract: | Sclerotinia sclerotiorum is one of the most devastating soil-inhabiting fungal plant pathogens infecting various crop plants including chickpea. Genetic
diversity of 24 isolates of S. sclerotiorum representing 10 different states of India was determined by different molecular markers and mycelial compatibility grouping
(MCG). The majority of the isolates showed more than 90% genetic similarity. Unweighted paired group method with arithmetic
average cluster analysis of DNA profiles generated by 21 RAPD primers grouped the isolates into seven categories showing high
magnitude of genetic homogeneity and showed partial correlation with geographical origin of the isolates. Identical ITS-RFLP
profiles were generated in all the isolates. Limited variability was observed among the nucleotide sequences of ITS region
of the isolates. The phylogenetic tree generated from bootstrap neighbor-joining analysis indicated that 50% of Indian populations
were distinct and grouped separately. The isolates were variable in mycelial compatibility and they were grouped into seven
MCGs, namely, MCG A, MCG B, MCG C, MCG D, MCG E, MCG F and MCG G. |
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