Effect of pH on protease activities in the large intestine |
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| Authors: | GT Macfarlane C Allison GR Gibson |
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| Institution: | Medical Research Council, Dunn Clinical Nutrition Centre, 100 Tennis Court Road, Cambridge CB2 1QL, UK |
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| Abstract: | The influence of pH on proteolysis in different fractions of human faeces was studied with a variety of chromogenic substrates. The pH optima of proteases in the washed cell fraction and washed particulate fraction were neutral to alkaline, whereas extra-cellular proteolysis in the cell-free supernatant fraction was relatively insensitive to pH over the range 6·4–8·0. Measurements with p -nitroanilide substrates suggested the presence of more than one elastase-like, trypsin-like and chymotrypsin-like protease in the gut. |
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