Whole Mounts of Small Embryos Attached Directly to Glass Slides |
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Authors: | Ivar P S Agrell |
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Institution: |
a Zoophysiological Institute, University of Lund, Lund, Sweden |
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Abstract: | Suspensions of sea urchin embryos spread over the surface of glass slides were attached to the glass by a rapid coagulation of the surface with alcohol. This was done either by dipping the glass slides into absolute alcohol or by a short exposure to alcohol vapor. Thereafter the slides were immediately transferred to the fixative. A suitable procedure includes fixation with Carnoy's fluid (alcohol, chloroform, acetic acid; 6:3:1) and staining with Gomori's hematoxylin after acid hydrolysis. |
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