Yiqi-Huoxue granule promotes angiogenesis of ischemic myocardium through miR-126/PI3K/Akt axis in endothelial cells |
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| Affiliation: | 1. Second School of Clinical Medicine, Henan University of Chinese Medicine, Zhengzhou 450002, China;2. Laboratory of Cell Imaging, Henan University of Chinese Medicine, Zhengzhou 450002, China;3. School of Graduate, Henan University of Chinese Medicine, Zhengzhou 450046, China;4. Institute of Cardiovascular Disease, Henan University of Chinese Medicine, Zhengzhou 450002, China;5. School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China;1. Department of Pharmacy, The Hospital Affiliated to Medical School of Yangzhou University (Taizhou People''s Hospital), Taizhou, China;2. State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China;3. Jiangsu Engineering Research Center of Cardiovascular Drugs Targeting Endothelial Cells, College of Health Sciences, School of Life Science, Jiangsu Normal University, Xuzhou, China |
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| Abstract: | BackgroundYiqi-Huoxue granule (YQHX), consisting of four kinds of traditional Chinese medicine, is an empirical prescription for the treatment of coronary heart disease. It is known to promote angiogenesis, but the mechanism is unknown.PurposeThis article investigates the possible mechanism of YQHX inducing angiogenesis in the ischemic myocardium.MethodsEAhy.926 cells were treated with YQHX hypoxic cardiomyocyte-conditioned medium (YHMCM) and the levels of VEGF, CD34, and phosphorylation of PI3K/Akt were detected by western blotting. Also, the effects on endothelial tube formation and migration were observed. The level of miR-126 was detected by qRT-PCR.ResultsYQHX promoted tube formation and migration of EAhy.926 cells and upregulated VEGF, CD34, and the phosphorylation of PI3K/AKT via regulating miR-126 levels. However, these effects were inhibited by a miR-126 inhibitor.ConclusionIn summary, YQHX improves angiogenesis by regulating the miR-126/PI3K/Akt signaling pathway, which indicates that YQHX could be a promising therapeutic strategy for ischemic myocardium. |
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