Rosmarinus officinalis essential oil modulates renal toxicity and oxidative stress induced by potassium dichromate in rats |
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| Institution: | 1. Department of Preventive Veterinary, Faculty of Veterinary, Federal University of Pelotas, Pelotas/RS, Brazil;2. Department of Veterinary Clinics, Faculty of Veterinary, Federal University of Pelotas, 96010-900 Pelotas/RS, Brazil;3. Post-Graduate Program of Pharmaceutical Sciences, Federal University of Rio Grande do Sul, 90610-000 Porto Alegre/RS, Brazil;4. Department of Organic Chemistry, Institute of Chemistry and Geoscience, Federal University of Pelotas, 96010-900 Pelotas/RS, Brazil;5. Department of Animal Pathology, Faculty of Veterinary, Federal University of Pelotas, 96010-900 Pelotas/RS, Brazil;6. Department of Pharmacology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), 90050-170 Porto Alegre/RS, Brazil |
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| Abstract: | BACKGROUNDChromium hexavalent (CrVI) is known as a toxic contaminant that induced oxidative stress and nephrotoxicity in humans and animals. Rosmarinus officinalis is a perennial herb rich in biologically active constituents that have powerful antioxidant properties. So, the current work evaluated the effectiveness of Rosmarinus officinalis essential oil (REO) against alterations induced by potassium dichromate in the kidney of male rats.METHODSGC-MS analysis, in vitro total phenol contents, and DPPH scavenging activity of REO were estimated. Thirty-five Wistar male rats were categorized into 5 groups. The first group was the control, the second one was orally administered rosemary essential oil (REO; 0.5 mL/kg BW), the third group was injected intraperitoneally with hexavalent chromium (CrVI; 2 mg/kg BW) for 14 days, the fourth group used as the protective group (REO was administrated 30 min before i.p. injection of CrVI) and the fifth group applied as the therapeutic group (rats injected with CrVI 30 min followed by oral administration of REO), respectively.RESULTSTwenty-nine components were detected with high total phenolic contents and high DPPH scavenging activity. Results revealed that CrVI- intoxicated rats showed a valuable increase in oxidative stress profile (TBARS and H2O2) and a notable decline in glutathione (GSH), total protein content, and enzymatic antioxidants (SOD, CAT, GPx, and GST). Furthermore, serum kidney functions biomarkers (urea, creatinine, and uric acid) were increased significantly. Also, the administration of CrVI showed histological and immunohistochemical (PCNA-ir) changes in rat kidney tissue. Otherwise, administration of REO pre or post-treatment with CrVI significantly restored most of the biochemical parameters in addition to improvement in kidney tissue architecture. Moreover, individual intake with REO exhibited an amendment in oxidative stress markers.CONCLUSIONConclusively, REO had a potential antioxidant capacity in ameliorating K2Cr2O7-induced nephrotoxicity, especially in the protection group. |
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| Keywords: | Chromium hexavalent rosemary essential oil oxidative stress antioxidant enzymes nephrotoxicity |
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