Value of models for membrane budding |
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| Institution: | 1. Department of Mechanical and Aerospace Engineering, University of California San Diego Jacobs School of Engineering, 9500 Gilman Drive #0411, La Jolla, CA, 92093, USA;2. Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, 94720, USA;1. Department of Chemistry, Institute for Biophysical Dynamics, James Franck Institute and Computation Institute, The University of Chicago, 5735 S Ellis Avenue, Chicago, IL 60637, USA;2. Institut Curie, Centre de Recherche, F-75248 Paris, France;3. Université Paris Diderot, F-75205 Paris, France;4. CNRS, Matière et Systèmes Complexes, UMR 7057, F-75205 Paris, France;5. CNRS, PhysicoChimie Curie, UMR 168, F-75248 Paris, France;6. Université Pierre et Marie Curie, F-75252 Paris, France;1. Cell Death and Metabolism, Center for Autophagy, Recycling and Disease, Danish Cancer Society Research Center, Copenhagen, Denmark;2. Orphazyme A/S, Copenhagen, Denmark;3. Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark |
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| Abstract: | The budding of membranes and curvature generation is common to many forms of trafficking in cells. Clathrin-mediated endocytosis, as a prototypical example of trafficking, has been studied in great detail using a variety of experimental systems and methods. Recently, advances in experimental methods have led to great strides in insights on the molecular mechanisms and the spatiotemporal dynamics of the protein machinery associated with membrane curvature generation. These advances have been ably supported by computational models, which have given us insights into the underlying mechanical principles of clathrin-mediated endocytosis. On the other hand, targeted experimental perturbation of membranes has lagged behind that of proteins in cells. In this area, modeling is especially critical to interpret experimental measurements in a mechanistic context. Here, we discuss the contributions made by these models to our understanding of endocytosis and identify opportunities to strengthen the connections between models and experiments. |
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| Keywords: | Clathrin-mediated endocytosis Bending modulus Helfrich Budding Snapthrough instability Membrane tension Multiscale modeling |
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