Neural peptide promotes the angiogenesis and osteogenesis around oral implants |
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| Institution: | 1. College of Pharmacy Jinan University, China;2. College of Traditional Chinese Medicine, China;3. Department of Traditional Chinese Medicine, First Affiliated Hospital of Jinan University, China;1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China;2. Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China;1. Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, 116044, China;2. Department of Clinical Biochemistry, College of Laboratory Medicine, Dalian Medical University, Dalian, China;3. Department of Clinical Laboratory, Second Affiliated Hospital of Dalian Medical University, Dalian, China;4. Department of Respirotory and Clinical Medecine, First Affiliated Hospital of Dalian Medical University, Dalian, China;1. School of Stomatology, Shandong University, Jinan, China;2. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, China;3. Department of Oral Implantology, The Affiliated Stomatology Hospital of Kunming Medical University, Kunming, China;4. Department of Stomatology, Jinan Central Hospital, Jinan, China;1. Bone Research Laboratory, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa;2. Department of Internal Medicine, School of Clinical Medicine, University of the Witwatersrand, Johannesburg, South Africa;3. Department of Oral Biological Sciences, School of Oral Health Sciences, University of the Witwatersrand, Johannesburg, South Africa |
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| Abstract: | Generally, impaired bones heal by bone repair and bone regeneration. These two processes are necessary during the healing period of dental implant. Vasculature plays a crucial role in bone healing because bones are highly vascularized tissue. Osteogenesis and angiogenesis are highly coupled processes and can be regulated by Hippo-YAP signaling pathway. Recent studies have demonstrated Hippo-YAP pathway may be regulated by alpha calcitonin gene-related peptide. However, the regulatory effects of αCGRP-YAP pathway on angiogenesis and osteogenesis during bone healing around implants remain unclear. Four groups of mice were established: KO Group: αCGRP −/− mice; KO + αCGRP group: αCGRP −/− mice with αCGRP overexpressing lentiviral transfection; KO + YAP group: αCGRP −/− mice with YAP overexpressing lentiviral transfection; WT group: wildtype mice. After 14 or 28 days, animals were sacrificed and tested. Results showed αCGRP deficiency hampered osteogenesis and angiogenesis. In addition, the impaired bone healing can be rescued by overexpressing αCGRP and YAP in αCGRP −/− mice. In-vivo results indicate αCGRP-YAP pathway promotes angiogenesis and osteogenesis in bone healing, especially at the early stage. Taken together, present study demonstrated αCGRP up-regulate the expression of YAP, and down-stream genes to promote the osteogenesis and angiogenesis around the implants. |
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