Salivary parameters alterations after early exposure to environmental methylmercury: A preclinical study in offspring rats |
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| Institution: | 1. Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Para, Belém, PA, Brazil;2. Laboratory of Molecular Pharmacology, Institute of Biological Sciences, Federal University of Para, Belém, PA, Brazil;1. Center for Advanced Studies and Technology (C.A.S.T.), \"G. d''Annunzio\" University of Chieti-Pescara, Via Luigi Polacchi 11, Chieti, I-66100, Italy;2. Department of Physics, Università Degli Studi di Milano, Via Celoria 16, Milano, I-20133, Italy;3. LASA, Department of Physics, Università Degli Studi di Milano and INFN-Milano, Via F.lli Cervi 201, Segrate, MI, I-20090, Italy;4. Institute of Clinical Immunotherapy and Advanced Biological Treatments, Piazza Pierangeli 1, Pescara, Rectorate of Leonardo da Vinci Telematic University, Largo San Rocco 11 Torrevecchia Teatina, CH, Italy;5. Department of Medicine and Aging Sciences, \"G. d''Annunzio\" University of Chieti-Pescara, via Luigi Polacchi 11, Chieti, I-66100, Italy;1. Institute of Keshan Disease, Chinese Center for Endemic Disease Control and Prevention, Harbin Medical University, Harbin 150081, China;2. Harbin Medical University, Harbin 150081, China;3. Heilongjiang Center for Disease Control and Prevention, Harbin 150030, China;4. The Second Institute for Endemic Disease Control and Prevention of Jilin Province, Jilin 132000, China;5. Institute of Endemic Disease Control and Prevention in Shandong Province, Jinan 250014, China;6. Sichuan Center for Disease Control and Prevention, Chengdu 610041, China;7. Institute for Endemic Disease Control and Prevention of Shaanxi Province, Xi’an 710082, China;8. Hubei Center for Disease Control and Prevention, Wuhan 430079, China;9. Tibet Autonomous Region Center for Disease Control and Prevention, Lhasa 850000, China;10. Yunnan Institute of Endemic Disease Control and Prevention, Dali 671000, China;11. Inner Mongolia Comprehensive Center for Disease Control and Prevention, Huhehot 010031, China;12. Chinese Center for Endemic Disease Control and Prevention, Harbin Medical University, Harbin 150081, China;1. Department of Physiology, School of Sport Sciences, University of Extremadura, Avenida de la Universidad s/n, 10003 Cáceres, Extremadura, Spain;2. Department of Sport Science, Faculty of Education, Pontifical University of Salamanca, C/Henry Collet, 52–70, CP: 37007 Salamanca, Spain;3. Movement, Brain and Health (MOBhe) Research Group, Center of Higher Education Alberta Giménez, affiliated to Comillas Pontifical University, Costa de Saragossa 16, 07013 Palma, Mallorca, Balearic Islands, Spain;4. Faculty of Language and Education, University of Nebrija, Campus La Berzosa, Calle del Hostal, 28248, Hoyo de Manzanares, Madrid, Spain;1. Univ. Grenoble Alpes, CEA, IRIG, 38000, Grenoble, France;2. Univ. Grenoble Alpes, INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA), and Environmental and System Biology (BEeSy), 38000, Grenoble, France;1. Center for Advanced Studies and Technology (C.A.S.T.), “G. d''Annunzio” University of Chieti-Pescara, Via Luigi Polacchi 11, Chieti, I-66100, Italy;2. Department of Physics, Università Degli Studi di Milano, Via Celoria 16, Milano, I-20133, Italy;3. LASA, Department of Physics, Università Degli Studi di Milano and INFN-Milano, Via F.lli Cervi 201, Segrate, MI, I-20090, Italy;4. Institute of Clinical Immunotherapy and Advanced Biological Treatments, Piazza Pierangeli 1, Pescara, Italy;5. Rectorate of Leonardo da Vinci Telematic University, Largo San Rocco 11 Torrevecchia, Teatina, CH, Italy;6. Department of Medicine and Aging Sciences, “G. d’Annunzio” University of Chieti-Pescara, via Luigi Polacchi 11, Chieti, I-66100, Italy;1. National Centre for Register-based Research, Department of Economics and Business, School of Business and Social Sciences, Aarhus University, Aarhus, Denmark;2. The Lundbeck Foundation Initiative for Integrative Psychiatric Research, iPSYCH, Aarhus and Copenhagen, Denmark;3. Centre for Integrated Register Based Research, CIRRAU, Aarhus University, Aarhus, Denmark;4. Department of Public Health, Section of Environment, Occupation and Health, Aarhus University, Aarhus, Denmark;5. Geological Survey of Denmark and Greenland, GEUS, Denmark;6. Big Data Centre for Environment and Health, BERTHA, Aarhus University, Aarhus, Denmark;7. Department of Geoscience, Aarhus University, Aarhus, Denmark |
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| Abstract: | BACKGROUNDMethylmercury (MeHg) is still considered a global pollutant of major concern; thus, it becomes relevant to investigate and validate alternative diagnostic methods to track early-life human exposure. This study aimed to evaluate the salivary parameters and to characterize potential mechanisms of oxidative damage on the salivary glands (SG) of offspring rats after pre- and postnatal environmental-experimental MeHg exposure.METHODSPregnant Wistar rats were daily exposed to 40 μg/kg MeHg during both gestational and lactation periods. Then, the saliva of offspring rats was analyzed in terms of flow rate, amylase activity, and total protein concentration. The SG of the offspring rats were dissected to perform the oxidative biochemistry analyses of antioxidant capacity against peroxyl radicals (ACAP), lipid peroxidation (LPO), and nitrite levels.RESULTSExposure to MeHg significantly decreased the ACAP, increased LPO and nitrite levels, decreased salivary flow rate, amylase activity, and total protein concentration.CONCLUSIONSaliva analyses can predict damages induced by early-life MeHg exposure and may be used as an auxiliary diagnostic method. |
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| Keywords: | Toxicology Methylmercury Development Salivary glands Saliva |
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