Hitting the sweet spot with capillary electrophoresis: advances in N-glycomics and glycoproteomics
Institution:
1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands;2. Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, The Netherlands;1. Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, Faculty of Engineering, University of Pannonia, Veszprem, Hungary;2. Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Centre for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary;3. Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic, Brno, Czech Republic;1. Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0760, USA;2. Bioinformatics and Systems Biology Graduate Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0760, USA;3. Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA;1. Horváth Csaba Memorial Laboratory for Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Egyetem ter 1, Hungary;2. Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Egyetem ut 10, Hungary
Abstract:
CE–MS glycoproteomics and glycomics. In intact methods, the glycans are identified while attached to the entire protein backbone, exposing the glycosylation profile and providing information on the macro-heterogeneity and site occupancy. In middle-up and bottom-up analysis, the protein is digested into sububits or smaller peptide fragments, revealing specific glycoforms and elucidating micro-heterogeneity in a site-specific approach. In released glycans techniques, the glycans are completely released from the protein molecule through chemical or enzymatic means. Unlike the middle-up and bottom-up techniques, released glycans do not offer site-specific information, but can achieve excellent levels of sensitivity in glycan microheterogeneity identification.
