The effects of two types of Western diet on the induction of metabolic syndrome and cardiac remodeling in obese rats |
| |
| Institution: | 1. Department of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil;2. Department of Patology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil;3. Department of Biostatistics, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil;1. Division of Nutritional Sciences, University of Illinois at Urbana-ChampaignUrbana, IL United States;2. Food Science and Human Nutrition, University of Illinois at Urbana-Champaign Urbana, IL United States;3. School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign Urbana, IL United States;4. Dorothy M. Davis Heart and Lung Research Institute, Department of Physiology and Cell Biology, The Ohio State University Wexner Medical Center, Columbus, OH, USA;5. Illinois Informatics Institute, University of Illinois at Urbana-Champaign, Urbana, IL United States;1. Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Yamagata University, Yamagata, Japan;2. Department of Immunology, Faculty of Medicine, Yamagata University, Yamagata, Japan;3. Department of Pathological Diagnostics, Faculty of Medicine, Yamagata University, Yamagata, Japan;4. Physical Chemistry for Life Science Laboratory, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan;5. AMED-CREST, Japan Agency for Medical Research and Development, Chiyoda-ku, Tokyo, Japan;6. Miyata Diabetes and Metabolism Clinic, Fukushima-ku, Osaka, Japan;1. College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, China;2. Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, China;1. Laboratory of Investigation in Chronic Diseases - LIDoC, Department of Physiological Sciences, Centre of Biological Sciences, Federal University of Santa Catarina - UFSC, Florianópolis, Santa Catarina, Brazil;2. Multicentre Graduate Program in Physiological Sciences, Centre of Biological Sciences, Federal University of Santa Catarina - UFSC, Florianópolis, Santa Catarina, Brazil;3. Graduate Program in Nutrition, Centre of Health Sciences, Federal University of Santa Catarina - UFSC, Florianópolis, Santa Catarina, Brazil;4. Department of Physiology and Pathology, School of Dentistry, São Paulo State University - UNESP, Araraquara, São Paulo, Brazil;1. Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Friedman School of Nutrition Science and Policy, Tufts University, Boston, Massachusetts, USA;2. USDA, ARS, Beltsville Human Nutrition Research Center, Diet Genomics and Immunology Laboratory, Beltsville, Maryland, USA;3. Laboratory of Mucosal Barrier Pathobiology, Department of Pathology, Brigham and Woman''s Hospital and Harvard Medical School, Boston, Massachusetts, USA;4. Section of Preventive Medicine and Epidemiology, Boston University School of Medicine, Boston, Massachusetts, USA;5. Intercollege Graduate Degree Program in Physiology and Department of Nutritional Science, Pennsylvania State University, University Park, Pennsylvania, USA |
| |
| Abstract: | Metabolic syndrome (MetS) include obesity as a critical feature and is strongly associated with risk of cardiovascular disease (CVD). Insights into mechanisms involved in the pathophysiology of these clinical manifestations are essential for the development of therapeutic strategies. Thus, Western diets (WD) have been widely employed in diet-induced obesity (DIO) model. However, there are variations in fat and sugar proportions of such diets, making comparisons challenging. We aimed to assess the impact of two types of the WD on metabolic status and cardiac remodeling, to achieve a DIO model that better mimics the human pathogenesis of MetS-induced CVD. Male Wistar rats were distributed into three groups: control diet, Western diet fat (WDF), and Western diet sugar (WDS) for 41 weeks. Metabolic and inflammatory parameters and cardiac changes were characterized. WDF and WDS feeding promoted higher serum triglycerides, glucose intolerance, and insulin resistance, while just WDF presented inflammation in adipose tissue. WDF-fed rats showed increased catalase activity and malondialdehyde (MDA) and carbonyl protein levels, suggesting cardiac oxidative stress, while WDS-fed rats only raised MDA. Both WD equally elevated protein expressions involved in lipid metabolism, but only WDF downregulated the glycolysis pathway. Furthermore, the mechanical myocardial function was impaired in obese rats, being more relevant in WDF. In conclusion, both WD effectively triggered MetS features, although inflammation was detected just on the WDF-fed animals. Moreover, the WDF promoted a more pronounced functional, metabolic, and oxidative cardiac disorder, suggesting to be an adequate model for studying CVD in the scenario of MetS. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
