Hsa_circ_0069094 accelerates cell malignancy and glycolysis through regulating the miR-591/HK2 axis in breast cancer |
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| Institution: | 1. Department of General Surgery, Wenzhou People’s Hospital, Wenzhou No. 3 Clinical Institute of Wenzhou Medical University, Wenzhou, Zhejiang Province, 325000, China;2. Department of Oncology, Lishui Central Hospital, Lishui, Zhejiang Province, 323000, China;3. The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200433, China;1. Department of Oncology, Chinese PLA General Hospital, Chinese PLA Medical School, Beijing, 100853, China;2. Department of Oncology, Fourth Medical Center of Chinese PLA General Hospital, Beijing, 100037, China;3. Department of Oncology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100071, China;1. School of Pharmaceutical Sciences, Jilin University, Changchun, China;2. Department of Biochemistry, College of Basic Medical Sciences, Jinlin University, Changchun, China;3. Changchun Vocational Institute of Technology, Changchun, China |
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| Abstract: | Circular RNAs (circRNAs) are implicated in the initiation and advancement of diverse tumors. CircRNA hsa_circ_0069094 (circ_0069094) has been reported to be upregulated in BC, but the biological role of circ_0069094 in BC is indistinct. Hence, we aimed to survey the biological role of circ_0069094 in BC. In the present study, we verified that circ_0069094 was upregulated in BC tissues and cells. BC patients with high circ_0069094 expression had a poor prognosis. Functional analysis revealed that circ_0069094 silencing induced apoptosis, curbed proliferation, and reduced glycolysis in BC cells in vitro, but circ_0069094 overexpression had an opposing influence. Also, circ_0069094 knockdown reduced BC growth in vivo. Mechanically, circ_0069094 was validated as a decoy for miR-591, which targeted HK2. Importantly, circ_0069094 sponged miR-591 to regulate HK2 expression. Both miR-591 silencing and HK2 overexpression counteracted circ_0069094 inhibition-mediated influence on cell proliferation, apoptosis, and glycolysis in BC cells. In conclusion, these results indicated that circ_0069094 facilitated cell malignancy and glycolysis by upregulating HK2 through adsorbing miR-591, suggesting that circ_0069094 might be a prognostic biomarker and therapeutic target for BC. |
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