Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio) |
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| Institution: | 1. Linus Pauling Institute, Oregon State University, Corvallis, Oregon;2. Integrative Biology Program, Oregon State University, Corvallis, Oregon;3. Molecular and Cell Biology Program;4. Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon;5. School of Biological and Population Health Sciences, Oregon State University, Corvallis, Oregon;1. Instituto de Fisiología Experimental (IFISE)-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, Argentina;2. Área Morfología, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, Argentina;3. CAECIHS, Universidad Abierta Interamericana, Rosario, Argentina;1. National Institute of Nutrition, Indian Council of Medical Research, Hyderabad, India;2. Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway;1. Laboratorio de Fisiología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos (CEFyBO-UBA/CONICET). Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina;2. Departamento de Química Biológica. Intendente Güiraldes, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina;3. Instituto de Química Biológica. Intendente Güiraldes, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina;1. Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt;2. Clinical Pharmacology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt |
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| Abstract: | An evaluation of the impact of vitamin E deficiency on expression of the alpha-tocopherol transfer protein (α-TTP) and related CRAL_TRIO genes was undertaken using livers from adult zebrafish based on the hypothesis that increased lipid peroxidation would modulate gene expression. Zebrafish were fed either a vitamin E sufficient (E+) or deficient (E–) diet for 9 months, then fish were euthanized, and livers were harvested. Livers from the E+ relative to E– fish contained 40-times more α-tocopherol (P <0.0001) and one fourth the malondialdehyde (P = 0.0153). RNA was extracted from E+ and E– livers, then subject to evaluation of gene expression of ttpa and other genes of the CRAL_TRIO family, genes of antioxidant markers, and genes related to lipid metabolism. Ttpa expression was not altered by vitamin E status. However, one member of the CRAL_TRIO family, tyrosine-protein phosphatase non-receptor type 9 gene (ptpn9a), showed a 2.4-fold increase (P = 0.029) in E– relative to E+ livers. Further, we identified that the gene for choline kinase alpha (chka) showed a 3.0-fold increase (P = 0.010) in E– livers. These outcomes are consistent with our previous findings that show vitamin E deficiency increased lipid peroxidation causing increases in phospholipid turnover. |
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