Anti-aging function and molecular mechanism of Radix Astragali and Radix Astragali preparata via network pharmacology and PI3K/Akt signaling pathway |
| |
| Affiliation: | 1. School of Food and biotechnological Engineering, Shaanxi University of Science and Technology, Xi''an, 710021, China;2. School of Chemistry and Chemical Engineering, Xi''an University of Science and Technology, Xi''an, 710054, China;1. Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan ROC;2. Department of Medicine Chest, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan ROC;3. Faculty of Pharmacy, School of Pharmaceutical Sciences, National Yang-Ming Chiao-Tung University, Taipei 11221, Taiwan ROC;4. Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan ROC;5. Department of Pharmacy, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ROC;6. Division of Rheumatology, Immunology and Allergy, Department of Internal Medicine, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 81342, Taiwan ROC;7. Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ROC;8. Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ROC;1. Department of Pancreatic Surgery, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuchang District, Wuhan 430060, Hubei Province, China;2. Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China;3. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, Hubei Province, China;4. Department of Cardiothoracic Surgery, General Hospital of Central Theater Command, Wuhan 430070, Hubei Province, China;1. College of Pharmacy, Engineering Research Center for Medicine, Harbin University of Commerce, Harbin, 150076, China;2. Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou, 57199, China;3. Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin, 150076, China;1. Guangzhou University of Chinese Medicine, China;2. Institute of Traditional Chinese Medicine Diagnostics, Hunan University of Chinese Medicine, China;3. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, China;4. The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, China;1. Smart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Republic of Korea;2. Natural Product Informatics Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do 25451, Republic of Korea;3. KHU-KIST Department of Converging Science and Technology, Graduate School Kyung Hee University, Seoul 130-701, Republic of Korea |
| |
| Abstract: | BackgroundRadix Astragali (RA) consists of the dried root of Astragalus membranaceus Bunge and is one of the most frequently used dietetic Chinese herbs to treat inflammation and neurodegenerative disease among other conditions. Radix Astragali preparata (RAP) is a medicinal form of RA. RA and RAP have been used as anti-aging agent, however, the mechanisms underlying their effects are still unclear.PurposeConsidering the wide application of RA and RAP in clinical practice, it is necessary to identify the better product between the two and elucidate the molecular mechanism responsible for their anti-aging effects.Study DesignIn this study, network pharmacology integrated with molecular biology techniques were employed to explore the possible mechanism of RA and RAP against aging.MethodsAging animal models were constructed by exposure to D-galactose (D-gal), and the anti-aging effect of RA and RAP were determined based on behavior tests and histomorphological observation. Network pharmacology was performed to construct the “compound-target-pathway” network. Gene and protein expression of possible targets were validated and analyzed using qRT-PCR and Western blotting.ResultsTreatment by RA and RAP could alleviate the symptoms of aging such as a decrease in body weight and organ indices, behavioral impairment, increased oxidative stress, weaken histopathological evaluation. The effect of RAP was more pronounced than that of RA in preventing aging process in a mouse model. The anti-aging effect of RA and RAP is associated with the balance of oxidative stress and activation of PI3K/Akt signaling pathway.ConclusionUsing an integrated strategy of network pharmacology and molecular biology we attempted to elucidate the mechanisms of action of RA and RAP. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
