Molecular identification and subtyping of Blastocystis sp. in hospital patients in Central China |
| |
| Affiliation: | 1. Statens Serum Institut, Copenhagen, Denmark;2. London School of Hygiene and Tropical Medicine, London, UK;1. Grupo de Investigaciones Microbiológicas, UR (GIMUR), Programa de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Bogotá, Colombia;2. Grupo de Parasitología, Instituto Nacional de Salud, Bogotá, Colombia;3. Programa Profesional de Biología, Grupo de Investigación en Parasitología y Microbiología Tropical, GIPAMT, Universidad Incca de Colombia, Bogotá, Colombia;4. Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia;5. Instituto de Investigaciones Biomédicas, Facultad de Medicina, Universidad Mayor de San Simón, Cochabamba, Bolivia;6. Laboratorio de Investigaciones FEPIS, Quininde, Esmeraldas Province, Ecuador;7. Facultad de Ciencias Médicas, de la Salud y la Vida, Universidad Internacional del Ecuador, Quito, Ecuador;8. Departamento de Parasitología del Hospital Nacional de Clínicas, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina;1. Department of Medical Microbiology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey;2. Department of Medical Microbiology, Faculty of Medicine, Erzincan University, Erzincan, Turkey;3. Department of Pediatric Intensive Care, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey;4. Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey |
| |
| Abstract: | Blastocystis is a common enteric protist that inhabits the gastrointestinal tract of approximately 1 billion people worldwide. In this study, a total of 1,070 patients from two hospitals in Zhengzhou, Central China were enrolled to know molecular characteristics of Blastocystis sp. The microorganism was identified and subtyped with a PCR amplification and sequencing of the small subunit ribosomal deoxyribonucleic acid (SSU-rDNA). The overall minimum prevalence of Blastocystis sp. in participants was 3.1% (33/1070). Although there were no significant differences on Blastocystis sp. infections among study sites, age groups, and gender, the higher infection was observed in the patients with gastrointestinal diseases (8.8%, 15/170). Sequence analysis of the 33 isolates revealed three known subtypes, such as ST1 (n = 7), ST3 (n = 23), and ST7 (n = 3). Among them, ST3 was the dominant subtype being detected in 23 isolates (69.7%), followed by ST1 (21.2%, 7/33) and ST7 (9.1%, 3/33). The phylogenetic analysis demonstrated that three subtypes (ST1, ST3 and ST7) were clustered with their reference sequences with good bootstrap support. The subtype determination of Blastocystis sp. isolates by the phylogenetic analysis was well supported by online platform. The present study provides the first molecular report of Blastocystis sp. infections in hospital patients in Central China. |
| |
| Keywords: | Molecular identification Subtyping Hospital patients |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
