Influence of Fe concentration in the medium on multicellular pollen grains and haploid plants induced by mannitol pretreatment in barley (Hordeum vulgare L.) |
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| Authors: | A Pulido F Bakos A Castillo M P Vallés B Barnabás A Olmedilla |
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| Institution: | (1) Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidin, Consejo Superior de Investigaciones Científicas, Granada;(2) Agricultural Research Institute, Hungarian Academy of Sciences, Martonvásár;(3) Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Zaragoza |
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| Abstract: | Summary. This study aims to clarify the short- and long-term effects of the iron concentration in the medium on androgenesis induced
in barley by isolated microspore culture. The ultrastructural features and pectin composition of the intine wall were studied
in the initial stages of androgenesis. The evolution of electron-dense iron deposits on the intine was analysed in multicellular
pollen grains obtained by isolated microspore culture performed for 3, 6, and 9 days using various concentrations of FeNa2 EDTA. Finally, the number of embryo-like structures and green plants obtained by microspore culture using different Fe concentrations
was evaluated in order to estimate the optimum concentration for isolated microspore culture.
Correspondence and reprints: Departamento de Bioquimica, Biologia Celular y Molecular de Plantas, Estación Experimental del
Zaidin, Consejo Superior de Investigaciones Cientificas, Profesor Albareda 1, 18008 Granada, Spain. |
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| Keywords: | : Androgenesis Culture medium Hordeum vulgare L Iron Microspore culture Pectin |
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