Bisphosphoglycerate mutase and pyruvate kinase activities during maturation of reticulocytes and ageing of erythrocytes |
| |
Authors: | J. Luque M. D. Delgado P. Rodriguez-Horche M. T. Company M. Pinilla |
| |
Affiliation: | (1) Departamento de Bioquimica y Biologia Molecular, Universidad de Alcalã de Henares, Madrid, Spain |
| |
Abstract: | An increase in bisphosphoglycerate mutase (BPGM) and a decrease in pyruvate kinase (PK), i.e. a decrease in PK/BPGM ratio, was observed in red cell populations from anemic rats containing 95% down to 3% reticulocytes in blood. Such a ratio has been used to study the fractionation of recticulocytes, according to their degree of maturation, after counter-current distribution of those cell populations in dextrahpoly (ethylene glycol) two-phase systems. When applying this procedure to the fractionation according to age of erythrocytes from normal rats, the decrease of PK with cellular age was observed without a significant variation in BPGM activity. |
| |
Keywords: | bisphosphoglycerate mutase pyruvate kinase maturation reticulocytes ageing erythrocytes counter-current distribution two-phase systems |
本文献已被 SpringerLink 等数据库收录! |