Some phytotoxic glycopeptides from Ceratocystis ulmi, the Dutch Elm Disease pathogen |
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Authors: | Gary Strobel Neal Van Alfen Kenneth D Hapner Michael McNeil Peter Albersheim |
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Institution: | 1. Department of Plant Pathology University, Bozeman, Mont. 59715, USA;2. Department of Chemistry, Montana State University, Bozeman, Mont. 59715, USA;3. Department of Biology, Utah State University, Logan, Utah 84321, USA;4. Department of Chemistry, University of Colorado, Boulder, Colo. 80302, U.S.A. |
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Abstract: | Ceratocystis ulmi, the causal agent of Dutch Elm Disease, produces phytotoxic glycopeptides in culture. A mixture of phytotoxic glycopeptides has been prepared by affinity chromatography on a concanavalin A-Sepharose column and collectively they have been termed the toxin. The polydisperse component that makes up the majority of the toxin (80%) by weight has a molecular weight of about 2.7·105. The large molecular weight component (<5%) elutes at the void volume of a Bio-Gel A50 m column. The other component (15%) appears as a trailing peak on the edge of the major component and has an approximate molecular weight of 7 · 104. The toxin is composed of 38% sugar residues, primarily rhamnose and mannose, and 7% amino acid residues. Methylation analysis coupled with mild acid hydrolysis indicates that the backbone of the polysaccharide portion of the toxin is composed of α-1,6-linked mannosyl residues with a 3-linked terminal rhamnosyl residue linked to C-3 of almost every mannosyl residue. The carbohydrate portion of the molecule is linked to the peptide via O-glycosidic linkages to both threonyl and seryl residues. All three components of the toxin are capable of causing wilt in stem cuttings of American elm. |
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