Department of Biological Chemistry, Division of Biology and Biomedical Sciences, Washington University, St. Louis, Mo. 63110 USA
Abstract:
One-to-one mercury complexes of thionicotinamide adenine dinucleotide (TNAD+) were prepared by using HgSO4 and Hg(CH3 COO?)2. Optical absorption spectroscopy indicated that the mercury probably binds to the TNAD+ through the thio-keto group on the pyridine ring. X-ray diffraction patterns of crysltals of mitichondrial malate dehydrogenase soaked in solution containing TNAD+ · mercury complex indicated binding and the X-ray intensity differences are different from mercurials alone.