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Glycogen synthesis in the fungus Neurospora crassa
Authors:Jose D Fontana  Clara R Krisman
Institution:Instituto de Investigaciones Bioquimicas “Fundación Campomar” and Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires Argentina
Abstract:An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of 14C]glucose from ADP-14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen glucosyltransferase were studied. The radioactive products obtained with UDP-14C]glucose or ADP-14C]glucose released all the radioactivity as maltose after α or β amylase treatment. Glucose 6-phosphate stimulated the synthetase when UDP-14C]glucose was the substrate but the stimulation was much greater with ADP-14C]glucose as glucosyl donor. Glucose 6-phosphate plus EGTA gave maximal stimulation. The system was completely dependent on the presence of a ‘primer’ of the α 1 → 4 glucan type.
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