Physicochemical properties of cartilage proteoglycans extracted by lanthanum chloride |
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| Authors: | Peter J Roughley Marion Hatt Roger M Mason |
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| Institution: | Department of Biochemistry, Charing Cross Hospital Medical School, University of London, Fulham Palace Road, London W6 8RF U.K. |
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| Abstract: | Bovine nasal cartilage was extracted with 0.5 M LaCl3 and the extract then diluted with nine volumes of water. The resulting precipitate (PLaCl3) contained the proteoglycan subunits, together with minor protein components, but was essentially free from hyaluronic acid. The properties of PLaCl3 were investigated by chemical analysis, electrophoresis, viscometry and analytical ultracentrifugation, and the results compared with those for proteoglycan obtained by caesium chloride density gradient centrifugation of 2 M CaCl2 cartilage extracts. Proteoglycan subunits (A1D1) prepared from PLaCl3 showed identical properties to those obtained from other high ionic strength cartilage extracts. |
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| Keywords: | To whom all correspondence should be addressed |
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