Demonstration of human pancreatic anionic trypsinogen in normal serum by radioimmunoassay |
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Authors: | Corey Largman James W. Brodrick Michael C. Geokas Janice H. Johnson |
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Affiliation: | 1. Enzymology Research Laboratory, Veterans Administration Hospital, Martinez, Calif. 94553, U.S.A.;2. Department of Medicine, University of California School of Medicine, Davis, Calif. 95616, U.S.A. |
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Abstract: | A specific radioimmunoassay for human pancreatic anionic trypsin has been developed. The trypsin employed as radioiodinated tracer in the assay was inactivated with tosyl-L-lysine chloromethyl ketone in order to prevent binding of the tracer to the serum inhibitors α1-antitrypsin and α2-macroglobulin. A normal serum level of immunoreactive anionic trypsin of 5.45 ng/ml was determined. The results of experiments in which serum was fractionated by Sephadex G-200 gel filtration suggest that essentially all of the immunoreactive material in normal human serum is trypsinogen. This finding implies that a small fraction of the zymogens synthesized in the pancreas are released directly into the circulation. |
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