| 依赖粪便材料的大熊猫肠道耶尔森氏菌的检测 |
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| 引用本文： | 杨水云,裴渭静,孙飞龙,陈曦,吴明宇,石晓强,吴晓民,任建设,贾康胜. 依赖粪便材料的大熊猫肠道耶尔森氏菌的检测[J]. 兽类学报, 2004, 24(2): 182-184 |
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| 作者姓名： | 杨水云 裴渭静 孙飞龙 陈曦 吴明宇 石晓强 吴晓民 任建设 贾康胜 |
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| 作者单位： | 西安交通大学生命科学与技术学院,西安,710049;陕西省动物研究所,西安,710032;陕西省珍稀野生动物抢救饲养研究中心,陕西周至,710400 |
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| 摘 要： | It is inevitable to develop noninvasive sampling methods to do studies on giant panda even diagnose the diseases since which is so endangered that it's impossible to carry out invasive sampling. A non-invasive sampling method to detect the intestinal pathogen, Yersirda enterocolitica in feces of pandas based designing PCR primers was established in this study. The main procedures are based on bacteria enrichment and cell lysis before binding the pathogen DNA to silica powder at high concentration of Kalium iodide and neutral pH conditions. Before PCR cycles, the binded DNA is washed with 80% ethanol and eluted with diluted EDTA buffer. Restdts showed that the silica-based feces DNA-purification method could remove the inhibitors of PCR so applicable to detect the target pathogen.
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| 关 键 词： | 大熊猫 小肠结肠炎耶尔森氏菌 粪便 非损伤性取样 |
| 文章编号： | 1000-1050(2004)02-0182-03 |
| 修稿时间： | 2003-04-16 |
Feces-based Detection of Yersinia enterocolitica in Intestine of Ailuropoda melanoleuca by PCR |
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| YANG ShuiyunPEI WeijingSUN FeilongCHEN XiWU MingyuSHI Xiaoqiang. Feces-based Detection of Yersinia enterocolitica in Intestine of Ailuropoda melanoleuca by PCR[J]. Acta Theriologica Sinica, 2004, 24(2): 182-184 |
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| Authors: | YANG ShuiyunPEI WeijingSUN FeilongCHEN XiWU MingyuSHI Xiaoqiang |
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| Abstract: | It is inevitable to develop noninvasive sampling methods to do studies on giant panda even diagnose the diseases since which is so endangered that it's impossible to carry out invasive sampling.A non-invasive sampling method to detect the intestinal pathogen, Yersinia enterocolitica in feces of pandas based designing PCR primers was established in this study. The main procedures are based on bacteria enrichment and cell lysis before binding the pathogen DNA to silica powder at high concentration of Kalium iodide and neutral pH conditions. Before PCR cycles, the binded DNA is washed with 80% ethanol and eluted with diluted EDTA buffer. Results showed that the silica-based feces DNA-purification method could remove the inhibitors of PCR so applicable to detect the target pathogen. |
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| Keywords: | Giant panda Yersinia enterocolitica Feces Non-invasive sampling |
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