Enhancement of phase separation by the addition of de-emulsifiers to three-phase (diesel oil/biocatalyst/aqueous phase) emulsion in diesel biodesulfurization |
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| Authors: | Choi Ok Kyoung Choi Kyung Suk Ryu Hee Wook Chang Yong Keun |
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| Institution: | (1) Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Daehyon-dong, Seodaemun-gu, Seoul, 120-750, Korea;(2) Department of Environmental and Chemical Engineering, Soongsil University, 1-1 Sangdo-5dong, Dongjak-gu, Seoul, 156-743, Korea;(3) Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Kusung-dong, Yusung-gu, Taejon, 305-701, Korea |
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| Abstract: | Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocatalyst recovered with ethanol addition showed similar specific growth rates (0.03 h–1) and dibenzothiophene desulfurization rates (6–7.2 mol l–1 h–1) to those (0.03 h–1 and 7.1 mol l–1, respectively) of the biocatalyst recovered with no ethanol addition. The desulfurization activity significantly increased as the number of the repeated recovery and reuse of the biocatalyst. |
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| Keywords: | biodesulfurization de-emulsifiers Gordonia nitida phase separation |
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