Teaching electron diffraction and imaging of macromolecules. |
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Authors: | W Chiu M F Schmid B V Prasad |
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Affiliation: | Werna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030. |
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Abstract: | Electron microscopic analysis can be used to determine the three-dimensional structures of macromolecules at resolutions ranging between 3 and 30 A. It differs from nuclear magnetic resonance spectroscopy or x-ray crystallography in that it allows an object's Coulomb potential functions to be determined directly from images and can be used to study relatively complex macromolecular assemblies in a crystalline or noncrystalline state. Electron imaging already has provided valuable structural information about various biological systems, including membrane proteins, protein-nucleic acid complexes, contractile and motile protein assemblies, viruses, and transport complexes for ions or macromolecules. This article, organized as a series of lectures, presents the biophysical principles of three-dimensional analysis of objects possessing different symmetries. |
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