Nucleotide substitutions in a yeast mitochondria cis-acting mutant located in the last intron of the apocytochrome b gene |
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| Authors: | C A Bonjardim F G Nóbrega |
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| Institution: | Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CP 20780, 01498 São Paulo SP, Brasil |
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| Abstract: | The region of mitochondrial DNA corresponding to the intron mutant M6-200 in Saccharomyces cerevisiae D273-10B has been isolated, and the nucleotide sequence of a 519 bp RsaI fragment has been determined. Three nucleotide substitutions were found at nucleotides +2650 (G----T), +2668 (G----A) and +2798 (A----G), all within the genetically defined location in the gene. Particular significance can be attributed to the first two changes (+2650 and +2668), that can be genetically isolated from the third substitution and, in addition, alter conserved sequence features detected in a study (1982) Biochimie 64, 867-881] of fungal mitochondrial introns. |
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| Keywords: | Mitochondrial intron mutant DNA sequence To whom correspondence should be addressed |
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