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Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis
Authors:A Mitrović  D Janošević  S Budimir  J Bogdanović Pristov
Institution:1. Institute for Multidisciplinary Research, University of Belgrade, Bulevar despota Stefana 142, Belgrade, 11000, Serbia
2. Institute of Botany and Botanical Garden ?Jevremovac“, University of Belgrade, Takovska 43, Belgrade, 11000, Serbia
3. Institute for Biological Research “Sini?a Stankovi?”, University of Belgrade, Bulevar despota Stefana 142, Belgrade, 11000, Serbia
Abstract:Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis from leaf explants were examined. During the early stages of shoot organogenesis there was a decrease in superoxide dismutase (SOD) and an increase in catalase (CAT) activity, and later during organogenesis there was an increase in peroxidase (POD) and polyphenol oxidase (PPO) activity. Two highly regulated turning points may be distinguished regarding activities and isoforms of antioxidative enzymes: the initiation of shoot organogenesis and the shoot bud formation. Our data suggest the role of specific CAT, POD, SOD and PPO isoforms in separate processes during T. bellus direct shoot organogenesis.
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