Micropropagation of Pinus peuce |
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Authors: | D Stojičić D Janošević B Uzelac V Čokeša S Budimir |
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Institution: | 1. Faculty of Science and Mathematics, University of Ni?, Vi?egradska 33, 18000, Ni?, Serbia 2. Institute of Botany and Botanical Garden, “Jevremovac” Faculty of Biology, University of Belgrade, Takovska 43, 11000, Belgrade, Serbia 3. Institute for Biological Research “Sini?a Stankovi?”, University of Belgrade, Bulevar despota Stefana 142, 11060, Belgrade, Serbia 4. Institute of Forestry, Kneza Vi?eslava 3, 11000, Belgrade, Serbia
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Abstract: | In Pinus peuce zygotic embryo culture grown on Gresshoff and Doy (1972; GD) basal medium, 2.22 μM benzyladenine (BA) was superior in promoting
adventitious bud induction during 4 weeks comparing to kinetin or BA + kinetin. Shoot elongation was achieved on half-strength
GD medium devoid of plant growth regulators and containing activated charcoal. Pulse treatment with 1 mM indole-3-butyric
acid (IBA) for 2 h, followed by transfer to half-strength GD medium, produced the most efficient rooting. Rooted shoots were
transplanted to the greenhouse and plantlets continued to grow and developed into phenotypically normal plants. Up to 10 plants
per explant can be obtained within 36 weeks from culture initiation. |
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