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Partial depolymerization of pectin by a photochemical reaction |
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| Authors: | Jankana Burana-osot Saori Hosoyama Toshihiko Toida |
| Affiliation: | a Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Silpakorn University, Nakorn-pathom 73000, Thailand b Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand c Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan d Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA |
| Abstract: | Complex heterogeneous polysaccharides that comprise pectin were partially depolymerized by a photochemical reaction using ultraviolet light in the presence of titanium dioxide catalyst. In a period of 6 h at pH 7, this UV/TiO2 process decreased the average molecular weight of pectin from 400 kDa to 200 kDa. The characterization of the partially depolymerized pectin, which was fractionated by size-exclusion chromatography, was performed by 1H NMR spectroscopy, and the spectra obtained showed that the resulting oligosaccharides and polysaccharides maintained the intact core structure of pectin. The monosaccharide content and depolymerization profile were determined by high-performance anion-exchange chromatography coupled with pulsed amperometric detection. This controlled photochemical depolymerization technique might be useful for preparation of pectin oligosaccharides as an ingredient in food and pharmaceutical products. |
| Keywords: | Pectin Photochemical depolymerization NMR spectroscopy HPAEC-PAD |
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