Synthesis of a trigalacturonic acid analogue mimicking the expected transition state in the glycosidases |
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| Authors: | Kazunori Yamamoto |
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| Affiliation: | Faculty of Agriculture and Life Science, Hirosaki University, 3-Bunkyo-cho, Hirosaki 036-8561, Japan |
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| Abstract: | A trigalacturonic acid analogue carrying a cyclohexene framework in place of the central pyranose ring was synthesized as a molecular probe for the mechanistic investigation of endo-polygalacturonase 1 (endo-PG 1). Preliminary enzymatic studies revealed that this analogue inhibited endo-PG 1 activity by about 30% at 0.3 mM concentration. |
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| Keywords: | endo-Polygalacturonase 1 Trigalacturonic acid analogue Transition state structure Cyclohexene ring |
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