Structural characterization of teichoic acids from Lactobacillus brevis |
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Authors: | Patricia M Sánchez Carballo Heikki Vilen Otto Holst |
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Institution: | a Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Germany b Department of Basic Veterinary Sciences, Division of Microbiology and Epidemiology, PO Box 66, FIN-00014 University of Helsinki, Helsinki, Finland |
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Abstract: | Teichoic acids are a major constituent of the cell wall of Gram-positive bacteria. Structural characterization of lipoteichoic and teichoic acids isolated from Lactobacillus brevis was undertaken using 1D and 2D NMR experiments as well as chemical methodology. Compositional analysis indicated the presence of high amounts of glycerol, glucose, and alanine. In the case of LTA octadecenoic acid was also detected. The basic LTA/WTA structure was identified as 1,3-poly(glycerol phosphate) nonstoichiometrically substituted at C-2 of the glycerol residues with d-Ala or α-d-Glc. In the case of LTA a higher amount of Ala could be detected and partial alanylation at position C-6 of the Glc could also be observed. |
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Keywords: | Lactobacillus brevis LTA structure WTA structure Alanylation NMR analysis Polyglycerol phosphate |
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