Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of oligosaccharides and oligosaccharide alditols obtained by hydrolysis of agaroses and carrageenans, two important types of red seaweed polysaccharides |
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Authors: | M.Kaniz Fatema,Diogo R.B. Ducatti,M. Eug?nia R. Duarte,Alberto S. Cerezo,Marí a C. Matulewicz |
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Affiliation: | a The United Graduate School of Agricultural Sciences, Faculty of Agriculture, Ehime University, 3-5-7-Tarumi, Matsuyama 790-8566, Japan b Plant Biophysics./Biochemistry Research Laboratory, College of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan c Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, Curitiba, Paraná, Brazil d Departamento de Quimica Organica—CIHIDECAR (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2-Ciudad Universitaria, 1428EGA Buenos Aires, Argentina |
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Abstract: | MALDI-TOF mass spectrometry analyses of several oligosaccharides (aldoses) and oligosaccharide alditols derived from agaroses, kappa- and iota-carrageenans using different matrices (2,5-dihydroxybenzoic acid, nor-harmane, ferulic acid, and the ionic liquid matrices 2,5-dihydroxybenzoic acid-n-butylamine and ferulic acid-n-butylamine) were conducted. These carbohydrates were selected as model compounds to study the MALDI prompt and post-source decay (PSD) fragmentation processes of both families of oligosaccharides. Sulfated alditols showed in the negative-ion mode the molecular ion as [M−Na]− together with the species yielded by their prompt fragmentation (mainly desulfation) while the sulfated oligosaccharides (aldoses) showed mainly glycosidic prompt fragmentation (glycosidic C-cleavages and desulfation). Non-sulfated aldoses and alditols, which could only be analyzed in positive-ion mode ([M+Na]+), did not suffer any prompt fragmentation. The former yielded cross-ring fragmentation in the PSD mode. Best results were obtained by using 2,5-dihydroxybenzoic acid and/or nor-harmane as matrices for all the compounds studied. |
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Keywords: | Agaro-oligosaccharides MALDI MS Agaro-oligosaccharide alditols MALDI MS Kappa-carrageenan oligosaccharides MALDI MS Iota-carrageenan oligosaccharide alditols MALDI MS Nor-harmane DHB |
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