Structural characterization of chondroitin sulfate from sturgeon bone |
| |
Authors: | Francesca Maccari Nicola Volpi |
| |
Institution: | a Department of Biology, University of Modena and Reggio Emilia, Modena, Italy b Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena, Italy |
| |
Abstract: | Chondroitin sulfate (CS) was purified for the first time from the bones of sturgeon and analyzed to evaluate its structure and properties. A single polysaccharide was extracted from sturgeon bone in a concentration of 0.28-0.34% for dry tissue and characterized as CS. By means of specific chondroitinases and HPLC separation of generated unsaturated repeating disaccharides, this polymer was found to be composed of ∼55% of disaccharide monosulfated in position 6 of the GalNAc, ∼38% of disaccharide monosulfated in position 4 of the GalNAc, and ∼7% of nonsulfated disaccharide. The charge density was 0.93 and the ratio of 4:6 sulfated residues was equal to 0.69, a value confirmed by 13C NMR experiments. Chondroitinase B confirmed that the purified sturgeon CS contained mainly GlcA (>99.5%) as uronic acid. PAGE analysis showed a CS having a high molecular mass with an average value of 39,880 according to HPSEC values producing a weight average molecular weight (Mw) of 37,500. On the basis of the data collected, it is reasonable to assume that CS isolated from sturgeon bone might be potentially useful for scientific and pharmacological applications, making this bony fish, which is generally discarded after ovary collection, a useful source of this polymer. Finally, this newly identified source of CS would enable the production of this macromolecule having a particular repeating disaccharide composition, structure, and biological properties. |
| |
Keywords: | CS chondroitin sulfate DS dermatan sulfate EULAR The European League Against Rheumatism GAG(s) glycosaminoglycan(s) IdoA l-idopyranosyluronic acid" target="_blank">α-l-idopyranosyluronic acid Mn number-average molecular weight Mw weight-average molecular weight Mz Z average molecular weight OA osteoarthritis SAX strong anion exchange SYSADOA Symptomatic Slow Acting Drug for OA ΔHexA l-threo-hex-4-enopyranosyluronic acid" target="_blank">4-deoxy-α-l-threo-hex-4-enopyranosyluronic acid |
本文献已被 ScienceDirect 等数据库收录! |
|