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A radioimmunoassay for corticosterone and its application to the measurement of stress in poultry. |
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| Authors: | R J Etches |
| Institution: | Department of Animal and Poultry Science University of Guelph Guelph, Ontario, Canada N1G 2W1 |
| Abstract: | A radioimmunoassay for corticosterone was developed using an antibody to corticosterone-21-hemisuccinate:bovine serum albumin. The assay possessed good specificity, sensitivity and reproducibility and required minimal sample preparation. Tests of adrenal function showed that stimulation of the adrenal with exogenous ACTH and with dexamethasone caused an increase and decrease, respectively, in plasma concentrations of corticosterone. Exposure to cold environmental temperatures caused an increase in plasma corticosterone. Handling and the removal of blood samples by venepuncture had no effect upon the concentration of corticosterone. It was concluded that this assay would accurately measure the response to stresses which affect the pituitary-adrenal axis. |
| Keywords: | 11β 21-dihydroxy-4-pregnene-3 20-dione corticosterone 4-pregnene-3 20-dione progesterone 21-hydroxy-4-pregnene-3 20-dione deoxycorticosterone 11β-hydroxy-4-pregnene-3 20-dione 11β-hydroxyprogesterone 1 3 5(10)-estratriene-3 17β-diol estradiol 17β-hydroxy-4-androstene-3-one testosterone 17α 21-dihydroxy-4-pregnene-3 20-dione 11-deoxycortisol 17α-hydroxy-4-pregnene-3 20-dione 17α-hydroxyprogesterone 11β 21-dihydroxy-4-pregnene-3 18 20-trione aldosterone 3β-hydroxy-5-pregnene-20-one pregnenolone 11β 17α 21-trihydroxy-4-pregnene 3 20-dione cortisol 9-flouro-16α-methyl-11β 17α 21-trihydroxy-1 4-pregnadiene-3 20-dione dexamethasone |
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