| 日本血吸虫中国大陆株28kDa GST基因在大肠杆菌中的表达 |
| |
| 引用本文： | 田锷,杨冠珍.日本血吸虫中国大陆株28kDa GST基因在大肠杆菌中的表达[J].动物学报,1996,42(4):421-427. |
| |
| 作者姓名： | 田锷 杨冠珍 |
| |
| 作者单位： | [1]中国农业科学院上海家畜寄生虫病研究所 [2]中国科学院上海生物化学研究所 |
| |
| 摘 要： | 在大肠杆菌ＴＢ１中表达含日本血吸虫中国大陆株２８ｋＤａ抗原基因的重组质粒，表达产物是融合蛋白，分子量来３３ｋＤａ。采用谷胱甘肽琼脂糖亲和层析柱纯化表达产物。２，４－二硝基氯苯／谷胱甘肽分光光度测定法和琼脂糖－淀粉凝胶电泳显示重组抗原具有较高的谷胱甘肽Ｓ－转移酶活力。
|
| 关 键 词： | 日本血吸虫 中国大陆株 谷胱甘肽S 转移酶 基因 |
EXPRESSION IN E. COLI OF 28kDa GLUTATHIONE S-TRANSFERASE GENE FROM CHINESE STRAIN OF SCHISTOSOMA JAPONICUM |
| |
| TIAN E,YANG GUAN-ZHEN,CAI YOU-MIN,SHEN WEI,SHI FU-HUI,WU XIANG-PU.EXPRESSION IN E. COLI OF 28kDa GLUTATHIONE S-TRANSFERASE GENE FROM CHINESE STRAIN OF SCHISTOSOMA JAPONICUM[J].Acta Zoologica Sinica,1996,42(4):421-427. |
| |
| Authors: | TIAN E YANG GUAN-ZHEN CAI YOU-MIN SHEN WEI SHI FU-HUI WU XIANG-PU |
| |
| Abstract: | This paper reports the expression of a recombinant plasmid which contains the 28kDa glutathione S-transferase (GST) gene of the Chinese strain of Schistosoma japonicum in Es- cherichia coli TB1. The product was a 33kDa fusion protein, which was purified by glu- tathione-agarose affinity chromatography. The purified recombinant antigen showed GST activity detected by 1-chloro-2, 4-dintrobenzene /glutathione spectrophotometry and a- garose-starch gel electrophoresis. The result of ELISA showed that the recombinant antigen could detect specific antibodies of sheep serum immunized with recombinant 28kDa GST or with native GST from the Chinese strain of Schistosoma japonicum, and the 33kDa molecule of recombinant antigen could be recognized by Western Blotting. All these results provide evidence that the recombinant antigen had GST antigenicity, and it would be assessed as a candidate vaccine in animal experiment against Schistosomiasis japonica. |
| |
| Keywords: | The Chinese strain of Schistosoma japonicum Glutathione S-Transterase Gene expression Enzyme activity Antigenicity |
| 本文献已被 CNKI 维普 等数据库收录！ |
