Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg. |
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| Authors: | V E Worrell D P Nagle Jr D McCarthy and A Eisenbraun |
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| Institution: | Department of Botany and Microbiology, University of Oklahoma, Norman 73019. |
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| Abstract: | A wild-type strain of Methanobacterium thermoautotrophicum Marburg was transformed by DNA from strains resistant to 5-fluorouracil. Recipient cells were grown without selection on gellan gum (GELRITE) plates with DNA. Drug-resistant cells were recovered by replica plating the resulting colonies onto drug plates. Transformation required high-molecular-weight DNA with appropriate markers and was not observed on agar or in liquid media under a variety of conditions. |
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