Reconstruction of the three-dimensional NMR spectrum of a protein from a set of plane projections |
| |
Authors: | Eriks Kupče Ray Freeman |
| |
Affiliation: | (1) Varian, Inc., Eynsham, Oxford, U.K;(2) Jesus College, Cambridge, U.K. |
| |
Abstract: | Three-dimensional protein NMR spectra can be obtained significantly faster than by traditional methods by a projection-reconstruction procedure related to X-ray tomography. First, two orthogonal projections are acquired in quick two-dimensional experiments with the evolution parameters t1 or t2 set to zero. These projections define a three-dimensional lattice; all cross-peaks must lie on this lattice but not all lattice points are occupied. A third experiment with t1 and t2 incremented simultaneously and in a fixed ratio, generates a projection onto a tilted plane and thus establishes the positions of all the cross-peaks unambiguously. This projection-reconstruction technique has been tested on the 500 MHz three-dimensional HNCO spectrum of ubiquitin. |
| |
Keywords: | HNCO projection-reconstruction three-dimensional NMR spectra ubiquitin |
本文献已被 PubMed SpringerLink 等数据库收录! |
|