Mapping candidate genes for oleate biosynthesis and their association with unsaturated fatty acid seed content in soybean |
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Authors: | Eleni Bachlava Ralph E. Dewey Joseph W. Burton Andrea J. Cardinal |
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Affiliation: | (1) Department of Crop Science, North Carolina State University, Campus Box 7620, Raleigh, NC 27695-7620, USA;(2) Present address: Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Rd., Athens, GA 30602, USA;(3) USDA-ARS, Soybean and Nitrogen Fixation Research Unit, 3127 Ligon St, Raleigh, NC 27607, USA |
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Abstract: | The development of high-oleate soybean germplasm is hindered by the lack of knowledge of the genetic factors controlling oleate phenotypic variation. In the present study, several candidate genes implicated in oleate biosynthesis were mapped and their cosegregation with oleate, linoleate and linolenate quantitative trait loci (QTLs) was investigated. FAD2-2C, a previously described ω-6 desaturase isoform, was localized on linkage group E; whereas, a novel FAD2-2 isoform, designated as FAD2-2D, mapped on linkage group N. In addition, two isoforms were identified for the aminoalcoholphosphotransferase-encoding GmAAPT1 gene, denoted AAPT1a and AAPT1b. A database query suggested that only one functional copy of the FAD6 gene, encoding a plastid localized ω-6 desaturase, exists in the soybean genome. AAPT1a and FAD6 mapped on linkage group D1b, 23.40 cM apart. Linolenate QTLs with minor effects were identified near the FAD6 and AAPT1a markers in two segregating populations. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Soybean Oleate biosynthesis QTL mapping Fatty acid content |
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