CHIP interacts with heat shock factor 1 during heat stress |
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Authors: | Kim Soo-A Yoon Jung-Hoon Kim Do-Kyung Kim Su-Gwan Ahn Sang-Gun |
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Affiliation: | Oral Biology Research Institute, Chosun University College of Dentistry, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, Republic of Korea. |
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Abstract: | Heat shock factor 1 (HSF1) is a major transactivator of heat shock genes in response to stress and mediates cell protection against various harmful conditions. In this study, we identified the interaction of CHIP (carboxyl terminus of the heat shock cognate protein 70-interacting protein) with the N-terminus of HSF1. Using GST full-down assay, we found that CHIP directly interacts with C-terminal deleted HSF1 (a.a. 1-290) but not with full-length HSF1 under non-stressed conditions. Interestingly, interaction of CHIP with full-length HSF1 was induced by heat shock treatment. The structural change of HSF1 was observed under heat stressed conditions by CD spectra. These observations demonstrate the direct interaction between HSF1 and CHIP and this interaction requires conformational change of HSF1 by heat stress. |
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Keywords: | HSF1, heat shock factor 1 CHIP, carboxyl terminus of the Hsc70-interacting protein Hsc70, heat shock cognate protein 70 Hsp90, heat shock protein 90 GST, glutathione S-transferase |
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