Isoenzyme analysis of Hammondia hammondi and Toxoplasma gondii sporozoites. |
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| Authors: | M L Dardé H Riahi B Bouteille M Pestre-Alexandre |
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| Institution: | Service de Parasitologie-Mycologie, CHU Dupuytren, Limoges, France. |
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| Abstract: | Isoenzyme analysis using isoelectrofocusing in polyacrylamide gels was used to distinguish Hammondia hammondi and Toxoplasma gondii sporozoites. Five enzyme systems were studied: aconitase (EC 4.2.1.3), aspartate aminotransferase (EC 2.6.1.1), glucose phosphate isomerase (EC 5.3.1.9), lactate dehydrogenase (EC 1.1.1.27), and phosphoglucomutase (EC 2.7.5.1). Three stocks of T. gondii belonging to 3 zymodemes were compared to 1 stock of H. hammondi. Hammondia hammondi differed from T. gondii at all 5 loci analyzed. This was observed for all 3 zymodemes of T. gondii. These results indicated clear genetic differences between the 2 species. |
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