Chlorogenic acid in a Nicotiana plumbaginifolia cell suspension |
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Affiliation: | 1. Department of Plant Science, Plant Sciences Complex, University of Pretoria, Hatfield 0083,South Africa;2. Swiss Tropical and Public Health Institute, Socinstrasse 57, CH 4002, Basel, Switzerland |
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Abstract: | A phenylpropanoid compound has been characterized in a Nicotiana plumbaginifolia cell suspension. This compound has been isolated and purified by semi-preparative reverse phase-high performance liquid chromatography. Its structure has been identified by NMR spectroscopy as 5-O-caffeoylquinic acid, which is chlorogenic acid (CA). The influence of culture conditions on the accumulation of this metabolite by N. plumbaginifolia cell suspensions has been studied. Darkness strongly inhibits the CA accumulation. Moreover, it has been shown that feeding experiments with caffeic acid had a deleterious effect upon the CA content. This one was not influenced by a supplementation with quinic acid. |
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