Phenolic profiles of untransformed and hairy root cultures of Plantago lanceolata |
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| Institution: | 1. UNESP - São Paulo State University/Coastal Campus of São Vicente, Laboratory of Bioprospection of Natural Products (LBPN), Pça Infante Dom Henrique S/N, 11330-900 São Vicente, SP, Brazil;2. Department of Biosciences, Federal University of São Paulo (UNIFESP), Silva Jardim Street, 136, Vila Mathias, 11015-020 Santos, SP, Brazil;3. Department of Exact and Earth Sciences, Federal University of São Paulo (UNIFESP), Campus Diadema, 09972-270 Diadema, SP, Brazil |
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| Abstract: | Untransformed root cultures and Agrobacterium rhizogenes induced root cultures (hairy roots) of Plantago lanceolata were investigated for caffeic acid glycoside esters, i.e. verbascoside (V) and plantamoside (P), by HPLC. Levels of V (6–12 mg.g–1 DW) and P (30–80 mg.g–1 DW) from untransformed and hairy root cultures were not modified by 0.1 mM (E)-cinnamic acid addition in Murashige and Skoog's culture medium. A part of the cinnamic acid was converted into (E)-p-coumaroyl-1-O-β-D-glucopyranoside, a phenolic derivative absent from control cultures without cinnamic acid. Maximum levels of this coumaroyl ester (6–8 mg.g–1 DW) were detected during 10 d and then slightly decreased from both root chemical profiles. |
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